Quantitative determination of melatonin in Lamium album flos Quantitative determination of melatonin in Lamium album flos

a HPlc method with dad detection for determination of melatonin in Lamium album flos was developed. The analysis was carried out on rP18 column using mixture of methanol and water (28:72 v/v) as a mobile phase. established calibration curve (r>0.9994), precision (rds values ranged from 0.5% to 1.5%), detection limit (0.025 μg/ml) and quantification limit (0.076 μg/ml) were found to be satisfactory for the proposed method. The determined content of melatonin in Lamium album flos was 0.15 μg/g of dry plant material.